Improved LC-MS/MS method development and validation for the determination of trandolapril and verapamil in human plasma
By: Yatha, Ravi
.
Contributor(s): Rajkamal, B.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol. 2(3).Description: 91-95p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective:
A simple, sensitive
and
rapid
LC
-MS/MS
technique
was developed for the quantitation
of
trandolapril (TDL) and
verapamil (VPL) in
a
biological
matrix
and validated
.
Method
s:
Sample preparation processed by SPE (Solid Phase
extraction
) on
phenomenex
cartridge using Ledipasvir as an internal standard.
Two
drugs were eluted on waters
symmetry
-RP
18
Result
s:
The quantification
approach
was validated in 5
-1500 ng
/ml
linear concentration
range
for TDL and 1
-2000 ng
/ml
for VPL. The
intraday
and
inter
-day
precision and accuracy
were
found to be
0.58% to 5.69% and 93% to 104% for two drugs. The average recoveries for TDL and VPL
were found to be 92.9% and 93.5% respectively.
(5μ,
15
0 mm
×4.
0 mm
) column with
the
mobile
composition of
10 mmol
ammonium formate
and
ACN(
acetonitrile
) in the ratio of 70:30
%V/V
. Detection and
quantitation
were
processed
by electrospray ionization in positive ionization mode.
Conclusio
n:
The developed work
was validated and can be applicable
to
the
routine
analysis of TDL and VPL simultaneously in
a biological
matrix
.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2018566 |
Objective:
A simple, sensitive
and
rapid
LC
-MS/MS
technique
was developed for the quantitation
of
trandolapril (TDL) and
verapamil (VPL) in
a
biological
matrix
and validated
.
Method
s:
Sample preparation processed by SPE (Solid Phase
extraction
) on
phenomenex
cartridge using Ledipasvir as an internal standard.
Two
drugs were eluted on waters
symmetry
-RP
18
Result
s:
The quantification
approach
was validated in 5
-1500 ng
/ml
linear concentration
range
for TDL and 1
-2000 ng
/ml
for VPL. The
intraday
and
inter
-day
precision and accuracy
were
found to be
0.58% to 5.69% and 93% to 104% for two drugs. The average recoveries for TDL and VPL
were found to be 92.9% and 93.5% respectively.
(5μ,
15
0 mm
×4.
0 mm
) column with
the
mobile
composition of
10 mmol
ammonium formate
and
ACN(
acetonitrile
) in the ratio of 70:30
%V/V
. Detection and
quantitation
were
processed
by electrospray ionization in positive ionization mode.
Conclusio
n:
The developed work
was validated and can be applicable
to
the
routine
analysis of TDL and VPL simultaneously in
a biological
matrix
.
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